Useful information for various sizes of cell culture dishes and flasks

There are various sizes of dishes and flasks used for cell culture. Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.

? Catalog No. Surface area (cm2) Seeding density Cells at confluency1 Versene
 (mL of 0.05% EDTA). Approx. volume		 Trypsin
 (mL of 0.05% trypsin, 0.53 mM EDTA). Approx. volume		 Growth medium
 (mL). Approx. volume
Dishes
35mm 150460; 150318 8.8 0.3 x 106 1.2 x 106 1 1 2
60mm 150462; 150288 21.5 0.8 x 106 3.2 x 106 3 3 5
100mm 150464; 150350 56.7 2.2 x 106 8.8 x 106 5 5 12
150mm 150468; 168381 145 5.0 x 106 20.0 x 106 10 10 30
Culture plates
6-well 140675 9.6 0.3 x 106 1.2 x 106 1 1 1 to 3
12-well 150628 3.5 0.1 x 106 0.5 x 106 0.4 to 1 0.4 to 1 1 to 2
24-well 142475 1.9 0.05 x 106 0.24 x 106 0.2 to 0.3 0.2 to 0.3 0.5 to 1.0
48-well 150687 1.1 0.03 x 106 0.12 x 106 0.1 to 0.2 0.1 to 0.2 0.2 to 0.4
96-well 167008 0.32 0.01 106 0.04 x 106 0.05 to 0.1 0.05 to 0.1 0.1 to 0.2
Flasks
T-25 156367; 156340 25 0.7 x 106 2.8 x 106 3 3 3–5
T-75 156499; 156472 75 2.1 x 106 8.4 x 106 5 5 8–15
T-175 159910; 159920 175 4.9 x 106 23.3 x 106 17 17 35–53
T-225 159934; 159933 225 6.3 x 106 30 x 106 22 22 45–68
1The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.


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