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Cell Culture Media Best Practices

Here are a few simple tips & tricks to help ensure your cell culture media is maintained for optimal performance.


If refrigerated, warm your cell culture media before use. Your cells would be cold at 2-8°C, and you would be too!


Keep your cell culture media protected from light. Light exposure degrades the essential vitamins in media that your cells need to grow.


Once you have supplemented your cell culture media with FBS, place the complete media into the refrigerator (2-8°C ) to maintain performance.


Use your supplemented media within 2-4 weeks to reduce the chances of contamination and the impact of pH drift.

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The Gibco Cell Culture Basics handbook and videos can help your lab attain reproducible results.

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