Welcome to the Thermo Scientific resource library. This page contains valuable links and supporting resources for standard molecular biology techniques, as well as specialty applications. It also provides easy access to a large variety of Thermo Scientific webinars, videos, articles, and more.

Learn more about our Thermo Scientific Molecular Biology Products

Spotlight articles

Articles highlighting key advances, technical tips, and new product capabilities

Workflow solutions for molecular biology

Links to help you find the right Thermo Scientific product for your specific project

Specific molecular biology applications

Information covering techniques including qPCR, mutagenesis, sequencing, epigenetics, in vitro transcription, genotyping, labeling, and detection

Webinars

Educational presentations focused on a variety of scientific techniques and solutions

Web tools

Online tools for analyzing primers, setting up reactions, and performing biochemical conversions and calculations

Thermo Scientific selection guides

PCR enzymes and master mixes
Reverse transcriptases
DNA ladders
PCR plastics

精選資源

在線工具

引物分析、反應體系建立、進行生化轉換及計算的在線工具.

焦點文章

瀏覽并學習我們關于關鍵進展、新產品及技術要訣和技巧的焦點文章.

選擇指南


Videos

Reverse transcription

Thermo Scientific Maxima First Strand cDNA Synthesis Kits with double-stranded DNase combine genomic DNA elimination and cDNA synthesis into a simple, one–tube workflow that can be completed in as few as 15 minutes.


PCR

In this video we will show you how to set up a PCR reaction using Thermo Scientific Phusion High-Fidelity DNA Polymerase.

How to video to demonstrate how to perform colony PCR as part of a cloning workflow using Thermo Scientific DreamTaq DNA Polymerase.

In this video, we will demonstrate how to amplify DNA directly from an unpurified sample using Thermo Scientific Direct PCR Kits. The master mix format enables robust PCR that can be completed in less than 30 minutes.


PCR plastics

This video explains different formats of PCR/qPCR plates and how to choose an optimal fit for your instrument.

This video demonstrates how to properly seal a PCR/qPCR plate with an adhesive film and cap strips.

This video reviews plate features important for robotic liquid handlers in high-throughput PCR/qPCR applications.

Thermo Scientific VersiPlate and VersiCap Mats are flexible consumables for PCR and qPCR making set-up and handling simple and easy.


Nucleic acid electrophoresis

Forget the Powder! Watch this how to video about new Thermo Scientific TopVision Agarose Tablets, which eliminate the need to weight out messy powdered agarose.

When running agarose gel electrophoresis, problems can arise from the following usage areas: improper gel preparation, overloading the gel, and inappropriate electrophoresis parameters. In this video, we will take a look at them one by one.


Molecular cloning

How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.

The Thermo Scientific CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any other blunt or sticky-end DNA fragment can be cloned.

Our Center of Excellence for Molecular Biology located in Vilnius, Lithuania is the world leader in the field of enzyme research and development, where over 30% of all known restriction enzymes were discovered/characterized.

Double digest your DNA in only 5-15 minutes with one buffer and load directly onto your gel with 176 Thermo Scientific FastDigest Enzymes.


Restriction and modifying enzymes

PCR相關資源

PCR相關文章

PCR在線工具

  • Tm計算器
  • 多重引物分析
  • Phusion的PCR反應體系建立
  • Phusion DNA聚合酶保真度計算器
PCR在線講座

在該在線講座中我們將討論DNA聚合酶技術的改進。我們將探討針對不同的PCR應用如何選擇最佳的DNA聚合酶,以及如何合理地組織PCR實驗流程.

該講座回顧了直接PCR方法的基本原理,介紹了最常見的實驗應用,同時提供了對于不同樣品類型應如何進行直接PCR的指南.

在該講座中,我們將回顧PCR的基礎知識,并對反應體系建立、反應優化、以及數據分析的最佳范例進行討論.

qPCR相關資源

qPCR在線工具

  • Tm計算器
  • 多重引物分析
  • 使用DyNAmo qPCR試劑盒進行反應體系建立
  • qPCR效率計算器

qPCR在線講座

在該在線講座中我們回顧了qPCR流程中最常見的變量及錯誤的來源,并討論了如何避免這些問題.

主題包括:qPCR原理及相關術語,檢測化學反應,擴增圖及Cq值,絕對定量vs.相對定量,以及檢測設計要素等.

限制性內切酶相關資源

限制性內切酶相關文章

  • 176種限制性內切酶,1種緩沖液

限制性內切酶在線工具

  • 雙酶切
  • 限制性內切酶搜索 (REsearch)

限制性內切酶在線講座

在該在線講座中我們將回顧與限制性內切酶消化相關的最常見問題,并給出創新性解決方案。我們還將為您介紹避免發生不完全酶切的要訣和技巧,并解釋出現的非預期的剪切模式及如何在克隆過程中以最佳方式使用RE.

反轉錄相關資源

反轉錄相關文章

反轉錄在線工具

  • 使用DyNAmo cDNA Synthesis Kit進行反應體系建立

核酸電泳及核酸純化相關資源

核酸電泳及純化相關文章

  • 忘掉那些瓊脂糖粉末吧!——Thermo Scientific TopVision瓊脂糖片劑

核酸電泳及核酸純化在線工具

  • OD轉換

核酸電泳及核酸純化在線講座

在該在線講座中我們將對植物研究課題進行概要性的介紹。我們將重點關注加速植物樣品處理的要訣和技巧,另外還將討論針對核酸純化和PCR的植物特殊解決方案.

在該講座中我們將討論核酸純化流程的主要步驟,包含從樣品收集到核酸保存等;并分享如何優化得率以及如何精確地對提取核酸的純度和濃度進行評估的技巧和建議.

分子克隆相關資源

分子克隆相關文章

  • Thermo Scientific克隆流程
  • Thermo Scientific的5種克隆捷徑
  • 分子克隆信息圖
  • 檢查T4 DNA連接酶活性
  • 篩選重組克隆的5種方法

分子克隆在線工具

  • 雙酶切
  • 限制性內切酶搜索 (REsearch)

分子克隆在線講座

在該在線講座中我們將對不同的DNA末端修飾方法、如何建立最優化的連接反應、如何評估并提高連接效率等等進行回顧。另外,我們還將討論傳統的T4 DNA連接酶克隆方法的替代方法,例如PCR克隆和LIC等.

該在線講座涵蓋了起始材料純化、插入片段及載體制備、DNA末端修飾、連接、轉移、以及陽性克隆分析篩選等多方面內容。建議在實驗流程中的每一步都增加必需的對照.

測序相關資源

測序在線講座

在該講座中我們將對常規的NGS文庫制備流程的每個步驟進行討論,同時引入一種全新的轉座子方法。另外還會介紹最佳范例以及重要的質量控制檢查點等.

測序相關文章

  • NGS文庫制備中的4大問題……以及如何解決這些問題

體外轉錄相關資料

體外轉錄相關文章

  • 使用T7來獲得高得率的RNA轉錄本